C. Michael Greenlief

C. Michael Greenlief
Associate Professor and Director
56 Chemistry
Research Emphasis: 

Analytical and Physical Chemistry, biological mass spectrometry, separation of complex mixtures, quantitative proteomics, directed metabolite analysis.


BS, Emporia State University, 1983
PhD, The University of Texas at Austin, 1987

Professional Experience: 

Director, MU NMR Facility, University of Missouri, 2007-present
Director, Charles W. Gehrke Proteomics Center, University of Missouri, 2008-present; Co-Director 2001-2008
Faculty member, Interdisciplinary Neuroscience Program, University of Missouri; 2018 – present
Office of Research Faculty Fellow, University of Missouri, 2004-2008
Director, Chemistry Mass Spectrometry Facility, 1997-present
Associate Professor, University of Missouri, 1994-present
Assistant Professor, University of Missouri, 1989-1994
Postdoctoral Research Associate, IBM T. J. Watson Research Center, 1987-1989

Professional Activities (partial listing of current activities)

American Chemical Society:

  • Secretary/Treasurer of local section 1992-93
  • Treasurer for the 1993 Midwest Regional Meeting
  • Program Chair for the 2003 Midwest Regional Meeting
  • General Chair for the 2014 Midwest Regional Meeting
  • Member of Midwest Region Board of Directors 1997-present, Board Secretary 2006, 2011, 2016 Board Chair-Elect 2007, 2012, 2017 Board Chair 2008, 2013, 2018
  • 2016 E. Ann Nalley Midwest Award for Volunteer Service
  • Member, National Committee on Minority Affairs (2020)
  • Member, ACS Scholar Selection Committee (2020)

Member, American Society for Mass Spectrometry, Society for the Advancement of Chicanos and Native Americans in Science, Society for Neuroscience

Editorial Board, PLoS One
Editorial Board, International Journal of Molecular Sciences
Editorial Board, Molecules


In my laboratory, experimental work is being conducted in the area of mass spectrometry. We develop new mass spectrometry-based methods to determine the bioactive components from dietary botanical supplements. We are also interested in biological systems at the protein level, as well as, quantitatively measuring targeted metabolites in a variety of matrices.

Select Publications: 

Grace Y. Sun, Michael K. Appenteng, Runting Li, Taeseon Woo, Bo Yang, Jiankun Cui, Chao Qin, Meixia Pan, Magdalena Cieślik, Jiankun Cui, Kevin L. Fritsche, Zezong Gu, Matthew Will, David Beversdorf, Agata Adamczyk, Xianlin Han, and C. Michael Greenlief, “Docosahexaenoic acid (DHA) supplementation alters phospholipid species and lipid peroxidation products in adult mouse brain, heart, and plasma,” NeuroMolecular Medicine, 2020, 1-12, doi.org/10.1007/s12017-020-08616-0.

Bo Yang, Runting Li, Pei N. Liu, Xue Geng, Brian P. Mooney, Chen Chen, Jianlin Cheng, Kevin L. Fritsche, David Q. Beversdorf, James C. Lee, Grace Y. Sun and C. Michael Greenlief, "Quantitative proteomics reveals docosahexaenoic acid-mediated neuroprotective effects in lipopolysaccharide-stimulated microglial cells," Journal of Proteome Research, 2020, 19: 2236-2246, doi.org/10.1021/acs.jproteome.9b00792.

Garrett Ungerer, Jiankun Cui, Tina Ndam, Mikeala Bekemeier, Hailong Song, Runting Li, Heather R. Siedhoff, Bo Yang, Michael K. Appenteng, C. Michael Greenlief, Dennis K. Miller, Grace Y. Sun, William R. Folk, and Zezong Gu, “Harpagophytum procumbens extract ameliorates allodynia and modulates oxidative and antioxidant stress pathways in a rat model of spinal cord injury,” NeuroMolecular Medicine, 2020, 22: 278-292, doi.org/10.1007/s12017-019-08585-z.

Hailong Song, Jiankun Cui, Valeri V. Mossine, C. Michael Greenlief, Kevin Fritsche, Grace Y. Sun, and Zezong Gu, “Bioactive components from garlic on brain resiliency against neuroinflammation and neurodegeneration (Review),” Experimental and Therapeutic Medicine, 2020, 19: 1554-1559, doi.org/10.3892/etm.2019.8389.

Xue Geng, Bo Yang, Runting Li, Tao Teng, Mary Jo Ladu, Grace Y. Sun, C. Michael Greenlief, and James C. Lee, “Effects of Docosahexaenoic Acid and its Peroxidation Product on Amyloid-β Peptide-Stimulated Microglia,” Molecular Neurobiology, 2020, 57:1085-1098, doi.org/10.1007/s12035-019-01805-4.

Danh C. Vu, Jihyun Park, Van K. Ho, Lloyd W. Sumner, Zhentian Lei, C. Michael Greenlief, Brian Mooney, Mark V. Coggeshall, and Chung-Ho Lin, “Identifying Health-promoting Bioactive Phenolics in Black Walnut Using a Cloud-based Metabolomics Platform,” Journal of Food Measurement and Characterization2020, 14: 770-777, doi.org/10.1007/s11694-019-00325-y.

Bo Yang, Kevin L. Fritsche, David Q. Beversdorf, Zezong Gu, James C. Lee, William R. Folk, C. Michael Greenlief, and Grace Y. Sun, “Ying-Yang mechanisms regulating lipid peroxidation of docosahexaenoic acid and arachidonic acid in the central nervous system,” Frontiers in Neurology, 2019, 10:642, doi.org/10.3389/fneur.2019.00642.

Bo Yang, Andrew L. Thomas, and C. Michael Greenlief, “Comparative proteomic analysis unveils critical pathways underlying the role of nitrogen fertilizer treatment in American elderberry,” Proteomes2019, 7:10, doi:10.3390/proteomes7010010.

Bo Yang, Runting Li, Taeseon Woo, Jimmy D. Browning, Jr., Hailong Song, Zezong Gu, Jiankun Cui, James C. Lee, Kevin L. Fritsche, David Q. Beversdorf, Grace Y. Sun and C. Michael Greenlief, “Maternal dietary docosahexaenoic acid alters lipid peroxidation products and (n-3)/(n-6) fatty acid balance in offspring mice,” Metabolites, 2019, 9: 40, doi.org/10.3390/metabo9030040.

Grace Y. Sun, Runting Li, Bo Yang, Kevin L. Fritsche, David Q. Beversdorf, Dennis Lubahn, Xue Geng, James C. Lee, and C. Michael Greenlief, “Quercetin potentiates docosahexaenoic acid to suppress lipopolysaccharide-induced oxidative and inflammatory responses in BV-2 microglial cells,” International Journal of Molecular Sciences, 2019, 20: 932, doi.org/10.3390/ijms20040932.

Hailong Song, Hui Zhou, Zhe Qu, Jie Hou, Weilong Chen, Weiwu Cai, Qiong Cheng, Dennis Y Chuang, Shanyan Chen, Shuwei Li, Jilong Li, Jianlin Cheng, C. Michael Greenlief, Yuan Lu, Agnes Simonyi, Grace Y Sun, Chenghan Wu, Jiankun Cui, and Zezong Gu, “From Analysis of Ischemic Mouse Brain Proteome to Identification of Human Serum Clusterin as a Potential Biomarker for Severity of Acute Ischemic Stroke,” Translational Stroke Research, 2019, 10: 546-556, doi.org/10.1007/s12975-018-0675-2.

Bo Yang, Runting Li, C. Michael Greenlief, Agnes Simonyi, Kevin L. Fritsche, Zezong Gu, Jiankun Cui, David Q. Beversdorf, and Grace Y. Sun, “Unveiling anti-oxidative and anti-inflammatory effects of 4-hydroxyhexenal and 4-hydroxynonenal and their regulation by docosahexaenoic acid and lipopolysaccharide in microglial cells,” Journal of Neuroinflammation2018, 15: 202, doi.org/10.1186/s12974-018-1232-3.

Grace Y. Sun, Agnes Simonyi, Kevin L. Fritsche, Dennis Y. Chuang, Mark Hannink, Zezong Gu, C. Michael Greenlief, Jeffrey Yao, James C. Lee, and David Q. Beversdorf, “Docosahexaenoic acid (DHA): an essential nutrient and a nutraceutical for brain health and diseases,” Prostaglandins, Leukotrienes, and Essential Fatty Acids, 2018, 136: 3-13, doi.org/10.1016/j.plefa.2017.03.006.

John-David Seelig, Tushar Ghosh, Nathan Jacobson, John Brockman, Luke Carter, C. Michael Greenlief, and Sudarshan K. Loyalka, “Sorption of Ag and its vaporization from graphite at high temperatures,” Journal of Nuclear Materials, 2017, 493: 132-146, doi.org/10.1016/j.jnucmat.2017.06.002.

Mitch C. Johnson, Matheus Dela Libera Tres, Andrew L. Thomas, George E. Rottinghaus, and C. Michael Greenlief, “Discriminant Analyses of Anthocyanin and Polyphenol Content of American Elderberry Juice from Multiple Environments Provide Genotype Fingerprint,” Journal of Agricultural and Food Chemistry2017, 65: 4044–4050, doi.org/10.1021/acs.jafc.6b05675.

Mitch C. Johnson, Hailong Song, Jiankun Cui, Valeri V. Mossine, Zezong Gu, and C. Michael Greenlief, “Method development and validation for quantitation of FruArg in mice plasma and brain tissue using UPLC-MS/MS,” ACS Omega, 2016, 1: 663-668, doi.org/10.1021/acsomega.6b00220.

Hailong Song, Yuan Lu, Zhe Qu, Valeri V. Mossine, Jie Hou, Jiankun Cui, Brenda Peculis, Thomas P. Mawhinney, Jianlin Cheng, C. Michael Greenlief, Kevin Fritsche, Frank J. Schmidt, Ronald B. Walter, Dennis B. Lubahn, Grace Y. Sun, and Zezong Gu, “Effects of aged garlic extract and FruArg on gene expression and signaling pathways in lipopolysaccharide-activated microglial cells,” Scientific Reports, 2016, 6: 35323, doi.org/10.1038/srep35323.

Zhe Qu, C Michael Greenlief, and Zezong Gu, “Quantitative proteomic approaches for analysis of protein S-nitrosylation,” Journal of Proteome Research, 2016, 15: 1-14, doi.org/10.1021/acs.jproteome.5b00857.

Mitch C. Johnson, Andrew L. Thomas, and C. Michael Greenlief, “Impact of Frozen Storage on the Anthocyanin and Polyphenol Contents of American Elderberry Fruit Juice,” Journal of Agricultural and Food Chemistry, 2015, 63: 5653-5659, doi.org/10.1021/acs.jafc.5b01702.

Jilong Li, Lin Sun, Kishore Banala, Jordan Maximillian Wilkins, Yuan Lu, Chad E. Niederhuth, Benjamin Ryan Merideth, Thomas P. Mawhinney, Valeri V. Mossine, C. Michael Greenlief, John C. Walker, William R. Folk, Mark Hannink, Dennis B. Lubahn, James A. Birchler, and Jianlin Cheng, “From Gigabyte to Kilobyte: A Bioinformatics Protocol for Mining Large RNA-Seq Transcriptomics Data,” PLoS ONE, 2015, 10 (4): e0125000, doi:10.1371/journal.pone.0125000.

Agnes Simonyi, Zihong Chen, Jinghua Jiang, Yijia Zong, Dennis Y. Chuang, Zezong Gu, Chi-Hua Lu, Kevin L. Fritsche, C. Michael Greenlief, Andrew L. Thomas, Dennis B. Lubahn and Grace Y. Sun, “Inhibition of Microglial Activation by Elderberry Extracts and Its Phenolic Components,” Life Sciences, 2015, 128: 30-38, doi: 10.1016/j.lfs.2015.01.037.

Hanzhi Wu, Mitch C. Johnson, Peter Lu, Kevin L. Fritsche, Andrew L. Thomas, Zongwei Cai and C. Michael Greenlief, “Determination of Anthocyanins and Total Polyphenols in a Variety of Elderberry Juices By UPLC/MS and Other Methods,” Acta Horticulturae, 2015, 1061: 43-51, doi: 10.17660/ActaHortic.2015.1061.3.

Hanzhi Wu, Mitch C. Johnson, Peter Lu, Kevin L. Fritsche, Andrew L. Thomas, Zongwei Cai and C. Michael Greenlief, “Peptidomics study in anthocyanin abundant juice of elderberry,” Talanta, 2015, 131: 640-644, doi.org: 10.1016/j.talanta.2014.08.022.

Research Division: