C. Michael Greenlief

C. Michael Greenlief
Associate Professor and Director
56 Chemistry
Research Emphasis: 

Analytical and Physical Chemistry, Biological mass spectrometry and separation of complex mixtures, Surface chemistry.


BS, Emporia State University, 1983
PhD, The University of Texas at Austin, 1987

Professional Experience: 

Director, MU NMR Facility, University of Missouri, 2007-present
Director, Charles W. Gehrke Proteomics Center, University of Missouri, 2008-present; Co-Director 2001-2008
Faculty member, Interdisciplinary Neuroscience Program, University of Missouri; 2018 – present
Office of Research Faculty Fellow, University of Missouri, 2004-2008
Director, Chemistry Mass Spectrometry Facility, 1997-present
Associate Professor, University of Missouri, 1994-present
Assistant Professor, University of Missouri, 1989-1994
Postdoctoral Research Associate, IBM T. J. Watson Research Center, 1987-1989

Professional Activities (partial listing of current activities)

American Chemical Society:

  • Secretary/Treasurer of local section 1992-93;
  • Treasurer for the 1993 Midwest Regional Meeting;
  • Program Chair for the 2003 Midwest Regional Meeting;
  • General Chair for the 2014 Midwest Regional Meeting;
  • Member of Midwest Region Board of Directors 1997-present, Board Secretary 2006, 2011, 2016 Board Chair-Elect 2007, 2012, 2017 Board Chair 2008, 2013, 2018
  • 2016 E. Ann Nalley Midwest Award for Volunteer Service

Member, American Society for Mass Spectrometry, American Vacuum Society, and the Society for the Advancement of Chicanos and Native Americans in Science

Editorial Board, PLoS One


In my laboratory, experimental work is being conducted in the areas of mass spectrometry and surface chemistry. We develop new mass spectrometry-based methods to determine the bioactive components from dietary botanical supplements. We are also interested in biological systems at the protein level. We create functional organic layers on semiconductor surfaces to isolate and bind biomolecules.

Select Publications: 

Grace Y. Sun, Runting Li, Bo Yang, Kevin L. Fritsche, Zezong Gu, Jiankun Cui, David Q. Beversdorf, Dennis Lubahn, James C. Lee, Andrew L. Thomas, and C. Michael Greenlief, “Quercetin potentiates docosahexaenoic acid to suppress lipopolysaccharide-induced oxidative and inflammatory responses in BV-2 microglial cells,” Life Sceinces, manuscript in preparation.

Bo Yang, Andrew L. Thomas, and C. Michael Greenlief, “Comparative proteomic analysis unveils critical pathways underlying the role of nitrogen fertilizer treatment in American elderberry,” Physiologia Plantarum, submitted (12/2018).

Bo Yang, Runting Li, Jimmy D. Browning, Kevin L. Fritsche, Hailong Song, Zezong Gu, Jiankun Cui, James C. Lee, David Q. Beversdorf, Grace Y. Sun, and C. Michael Greenlief, “Maternal dietary docosahexaenoic acid alters lipid peroxidation products and (n-3)/(n-6) fatty acid balance in offspring mice,” The Journal of Nutritional Biochemistry, revised (11/2018).

Hailong Song, Hui Zhou, Zhe Qu, Jie Hou, Weilong Chen, Weiwu Cai, Qiong Cheng, Dennis Y Chuang, Shanyan Chen, Shuwei Li, Jilong Li, Jianlin Cheng, C. Michael Greenlief, Yuan Lu, Agnes Simonyi, Grace Y Sun, Chenghan Wu, Jiankun Cui, and Zezong Gu, “From Analysis of Ischemic Mouse Brain Proteome to Identification of Human Serum Clusterin as a Potential Biomarker for Severity of Acute Ischemic Stroke,” Translational Stroke Research, 2018, xx:1-11, doi.org/10.1007/s12975-018-0675-2.

Bo Yang, Runting Li, C. Michael Greenlief, Agnes Simonyi, Kevin L. Fritsche, Zezong Gu, Jiankun Cui, David Q. Beversdorf, and Grace Y. Sun, “Unveiling anti-oxidative and anti-inflammatory effects of 4-hydroxyhexenal and 4-hydroxynonenal and their regulation by docosahexaenoic acid and lipopolysaccharide in microglial cells,” Journal of Neuroinflammation2018, 15: 202, doi.org/10.1186/s12974-018-1232-3.

Grace Y. Sun, Agnes Simonyi, Kevin L. Fritsche, Dennis Y. Chuang, Mark Hannink, Zezong Gu, C. Michael Greenlief, Jeffrey Yao, James C. Lee, and David Q. Beversdorf, “Docosahexaenoic acid (DHA): an essential nutrient and a nutraceutical for brain health and diseases,” Prostaglandins, Leukotrienes, and Essential Fatty Acids, 2018, 136: 3-13,  doi.org/10.1016/j.plefa.2017.03.006.

John-David Seelig, Tushar Ghosh, Nathan Jacobson, John Brockman, Luke Carter, C. Michael Greenlief, and Sudarshan K. Loyalka, “Sorption of Ag and its vaporization from graphite at high temperatures,” Journal of Nuclear Materials, 2017, 493: 132-146, doi.org/10.1016/j.jnucmat.2017.06.002.

Mitch C. Johnson, Matheus Dela Libera Tres, Andrew L. Thomas, George E. Rottinghaus, and C. Michael Greenlief, “Discriminant Analyses of Anthocyanin and Polyphenol Content of American Elderberry Juice from Multiple Environments Provide Genotype Fingerprint,” Journal of Agricultural and Food Chemistry2017, 65: 4044–4050, doi.org/10.1021/acs.jafc.6b05675.

Mitch C. Johnson, Hailong Song, Jiankun Cui, Valeri V. Mossine, Zezong Gu, and C. Michael Greenlief, “Method development and validation for quantitation of FruArg in mice plasma and brain tissue using UPLC-MS/MS,” ACS Omega, 2016, 1: 663-668, doi.org/10.1021/acsomega.6b00220.

Hailong Song, Yuan Lu, Zhe Qu, Valeri V. Mossine, Jie Hou, Jiankun Cui, Brenda Peculis, Thomas P. Mawhinney, Jianlin Cheng, C. Michael Greenlief, Kevin Fritsche, Frank J. Schmidt, Ronald B. Walter, Dennis B. Lubahn, Grace Y. Sun, and Zezong Gu, “Effects of aged garlic extract and FruArg on gene expression and signaling pathways in lipopolysaccharide-activated microglial cells,” Scientific Reports, 2016, 6: 35323, doi.org/10.1038/srep35323.

Zhe Qu, C Michael Greenlief, and Zezong Gu, “Quantitative proteomic approaches for analysis of protein S-nitrosylation,” Journal of Proteome Research, 2016, 15: 1-14, doi.org/10.1021/acs.jproteome.5b00857.

Mitch C. Johnson, Andrew L. Thomas, and C. Michael Greenlief, “Impact of Frozen Storage on the Anthocyanin and Polyphenol Contents of American Elderberry Fruit Juice,” Journal of Agricultural and Food Chemistry, 2015, 63: 5653-5659, doi.org/10.1021/acs.jafc.5b01702.

Jilong Li, Lin Sun, Kishore Banala, Jordan Maximillian Wilkins, Yuan Lu, Chad E. Niederhuth, Benjamin Ryan Merideth, Thomas P. Mawhinney, Valeri V. Mossine, C. Michael Greenlief, John C. Walker, William R. Folk, Mark Hannink, Dennis B. Lubahn, James A. Birchler, and Jianlin Cheng, “From Gigabyte to Kilobyte: A Bioinformatics Protocol for Mining Large RNA-Seq Transcriptomics Data,” PLoS ONE, 2015, 10 (4): e0125000, doi:10.1371/journal.pone.0125000.

Agnes Simonyi, Zihong Chen, Jinghua Jiang, Yijia Zong, Dennis Y. Chuang, Zezong Gu, Chi-Hua Lu, Kevin L. Fritsche, C. Michael Greenlief, Andrew L. Thomas, Dennis B. Lubahn and Grace Y. Sun, “Inhibition of Microglial Activation by Elderberry Extracts and Its Phenolic Components,” Life Sciences, 2015, 128: 30-38, doi: 10.1016/j.lfs.2015.01.037.

Hanzhi Wu, Mitch C. Johnson, Peter Lu, Kevin L. Fritsche, Andrew L. Thomas, Zongwei Cai and C. Michael Greenlief, “Determination of Anthocyanins and Total Polyphenols in a Variety of Elderberry Juices By UPLC/MS and Other Methods,” Acta Horticulturae, 2015, 1061: 43-51, doi: 10.17660/ActaHortic.2015.1061.3.

Hanzhi Wu, Mitch C. Johnson, Peter Lu, Kevin L. Fritsche, Andrew L. Thomas, Zongwei Cai and C. Michael Greenlief, “Peptidomics study in anthocyanin abundant juice of elderberry,” Talanta, 2015, 131: 640-644, doi.org: 10.1016/j.talanta.2014.08.022.

Hui Zhou, Zhe Qu, Valeri V. Mossine, Dineo L. Nknolise, Jilong Li, Zhenzhou Chen, Jianlin Cheng, C. Michael Greenlief, Thomas P. Mawhinney, Paula N. Brown, Kevin L. Fritsche, Mark Hannink, Dennis B. Lubahn, Grace Y. Sun, Zezong Gu, “Proteomic analysis of the effects of aged garlic extract and its FruArg component on lipopolysaccharide-induced neuroinflammatory response in microglial cells,” PLoS ONE, 2014,9 (11): e113531, doi: 10.1371/journal.pone.0113531.

Ping Gong, Zeynep Madak-Erdogan, Jilong Li, Jianlin Cheng, C. Michael Greenlief, William G. Helferich, John A. Katzenellenbogen and Benita S. Katzenellenbogen, “Transcriptome Analyses Reveal Gene Networks Regulated by ERα and ERβ that Control Distinct Effects of Different Botanical Estrogens,” Nuclear Receptor Signaling, 2014 12: 1-13, doi:10.1621/nrs.12001.

Jeremy Dahmen, Yongqiang Yang, C. Michael Greenlief, Gary Stacey, and Heather Hunt, “Interfacing whispering gallery mode optical microresonator biosensors with the plant defense elicitor chitin,” Colloids and Surfaces B: Biointerfaces, 2014, 122: 241-249, doi:10.1016/j.colsurfb.2014.06.067.

Zhe Qu, Fanjun Meng, Ryan Bomgarden, Rosa, Viner, Jilong Li, John Rogers, Jianlin Cheng, C. Michael Greenlief, Jiankun Cui, Dennis Lubahn, Grace Sun, and Zezong Gu, “Proteomic Quantification And Site-Mapping of S-Nitrosylated Proteins Using Isobaric iodoTMT Reagents,” Journal of Proteome Research, 2014, 13: 3200-3211, doi: 10.1021/pr401179v.

Zhe Qu, Fanjun Meng, Quanhui Wang, Fan Wei, Jilong Li, Jianlin Cheng, C. Michael Greenlief, Dennis B. Lubahn, Grace Y. Sun, Siqi Liu, and Zezong Gu, “NitroDIGE Analysis Reveals Inhibition of Protein S-Nitrosylation by Epigallocatechin Gallates in Lipopolysaccharide-stimulated Microglial Cells,” Journal of Neuroinflammation, 2014, 11:17, doi:10.1186/1742-2094-11-17.

Research Division: