C. Michael Greenlief
Associate Professor and Director
56 Chemistry
573-882-3288
Bio

Education: 

BS, Emporia State University, 1983
PhD, The University of Texas at Austin, 1987

Professional Experience: 

Program Director (rotator), Division of Chemistry, National Science Foundation (2023–present)
Director, MU NMR Facility, University of Missouri, 2007-present
Faculty member, Interdisciplinary Neuroscience Program, University of Missouri; 2018 – present
Director, Charles W. Gehrke Proteomics Center, University of Missouri, 2001-2023
Office of Research Faculty Fellow, University of Missouri, 2004-2008
Director, Chemistry Mass Spectrometry Facility, 1997-present
Associate Professor, University of Missouri, 1994-present
Assistant Professor, University of Missouri, 1989-1994
Postdoctoral Research Associate, IBM T. J. Watson Research Center, 1987-1989

Professional Activities (partial listing of current activities)

American Chemical Society:

  • Secretary/Treasurer of local section 1992-93
  • Treasurer for the 1993 Midwest Regional Meeting
  • Program Chair for the 2003 Midwest Regional Meeting
  • General Chair for the 2014 Midwest Regional Meeting
  • Member of Midwest Region Board of Directors 1997-present, Board Secretary 2006, 2011, 2016; Board Chair-Elect 2007, 2012, 2017; Board Chair 2008, 2013, 2018
  • 2016 E. Ann Nalley Midwest Award for Volunteer Service
  • Member, National Committee on Minority Affairs (2020-2024)
  • Member, ACS Scholars Committee (2020-2024)

Member, American Association for the Advancement of Science, American Society for Mass Spectrometry, Society for the Advancement of Chicanos and Native Americans in Science, Society for Neuroscience

Editorial Board, PLoS One (2018-2023)
Editorial Board, Molecules (2019-2023)
Topics Board Editor, International Journal of Molecular Sciences (2021-2023)
Editorial Board, International Journal of Molecular Sciences (2019-2021)

Research

Research Emphasis: 

Analytical and Physical Chemistry, biological mass spectrometry, separation of complex mixtures, quantitative proteomics, directed metabolite analysis.

Research: 

In my laboratory, experimental work is being conducted in the area of mass spectrometry and NMR. We develop new methods to determine the bioactive components from dietary botanical supplements. We are also interested in biological systems at the protein level, as well as, quantitatively measuring targeted metabolites in a variety of matrices.

Select Publications

Andrew L. Thomas, George E. Rottinghaus, Matheus Dela Libera Tres, Patrick L. Byers, John D. Avery, Jr., Martin Kaps, Igolf Gruen, Akash Mazumder, Diann M. Thomas, Megan N. Westwood, Giselle Campos, Richard Biagioni, Mitch C. Johnson, and C. Michael Greenlief, “Soil nitrogen fertility influences growth and yield of American elderberry, but is less impactful than genotype and environment on other horticulture characteristics,” HortScience, 2024, 56:787-793, doi.org/10.21273/hortsci17772-24.

Marcus Jackson, Shanyan Chen, Pei Liu, Martin Langenderfer, Chao Li, Heather R. Siedhoff, Ashley Balderrama, Runting Li, Catherine E. Johnson, C. Michael Greenlief, Ibolja Cernak, Ralph G. DePalma, Jiankun Cui, Zezong Gu, “Quantitative Proteomic Profiling in Brain Subregions of Mice Exposed to Open-Field Low-intensity Blast Reveals Position-Dependent Blast Effects,” Shock Waves, 2024, doi.org/10.1007/s00193-024-01169-2.

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Ashley F. Curtis, Madison Musich, Amy N. Costa, Joshua Gonzalez, Hyeri Gonzales, Bradley J. Ferguson, Andrew L. Thomas, Xing Wei, Pei Lu, Briana Kille, C. Michael Greenlief, Joel Shenker and David Q. Beversdorf, “Feasibility and preliminary efficacy of American elderberry juice for improving cognition and inflammation in patients with mild cognitive impairment,” International Journal of Molecular Sciences, 2024, 25:4352, doi.org/10.3390/ijms25084352.

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Kate Nyarko and C. Michael Greenlief, “Investigations of major ɑ-dicarbonyl content in US honey of different geographical origins,” Molecules, 2024, 29:1588, doi.org/10.3390/molecules29071588.

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Marcus Jackson, Shanyan Chen, Thao Thi Nguyen, Heather R. Siedhoff, Ashley Balderrama, Amitai Zuckerman, Runting Li, C. Michael Greenlief, Gregory Cole, Sally Frautschy, Jiankun Cui and Zezong Gu, “The chronic effects of a single low-intensity blast exposure on phosphoproteome networks and cognitive function influenced by mutant tau overexpression,” International Journal of Molecular Sciences, 2024, 25:3338, doi.org/10.3390/ijms25063338.

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Chao Li, Shanyan Chen, Heather Siedhoff, DeAna Grant, Pei Liu, Ashley Balderrama, Marcus Johnson, Amitai Zuckerman, C. Michael Greenlief, Firas H Kobeissy, Kevin W. Wang, Ralph G. DePalma, Ibolja Cernak, Jiankun Cui, and Zezong Gu, “Low-intensity open field blast exposure effects on neurovascular unit ultrastructure in mice,” Acta Neuropathologica Communications, 2023, 11:144, doi.org/10.1186/s40478-023-01636-4.

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K. S. Ravichandran, E.S. Silva, M. Moncada, P. Perkins-Veazie, M. A. Lila, C.M. Greenlief, A. L. Thomas, R. Hoskin, and K. Krishnaswamy, “Spray-drying to encapsulate bioactive compounds from juice and pomace of American elderberry using carbohydrate and protein carriers,” Food Bioscience, 2023, 55:102981, doi.org/10.1016/j.fbio.2023.102981.

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Katelyn B. Hartnett, Bradley J. Ferguson, Patrick Hecht, Luke E Schuster, Joel I. Shenker, David R. Mehr, Kevin L. Fritsche, Martha Belury, Douglas W. Scharre, Adam J. Horwitz, Briana Kille, Briann Sutton, Paul Tatum, C. Michael Greenlief and David Q Beversdorf, “Potential neuroprotective effects of dietary omega-3 fatty acids on stress in aging and Alzheimer’s disease,” Biomolecules, 2023, 13:1096, doi.org/10.3390/biom13071096.

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Kate Nyarko, Kaitlyn Boozer, and C. Michael Greenlief, “Profiling of the Polyphenol Content of Honey from Different Geographical Origins in the United States,” Molecules, 2023, 28: 5011, doi.org/10.3390/molecules28135011.

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Colleen L. Ray, James A. Gawenis, Madison Bylo, Jonny Pescaglia, and C. Michael Greenlief, “Detection of Vegetable Oil Adulteration in Pre-Grated Bovine Hard Cheeses Via 1H NMR Spectroscopy,” Molecules, 2023, 28: 920, doi.org/10.3390/molecules28030920.

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Colleen L. Ray, Madison Bylo, Jonny Pescaglia , James A. Gawenis, and C. Michael Greenlief, “Delta-8 tetrahydrocannabinol product impurities,” Molecules, 2022,  27: 6924, doi.org/10.3390/molecules27206924.

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Xing Wei, Pei N. Liu, Brian P. Mooney, Thao Thi Nguyen, and C. Michael Greenlief, “A comprehensive study of gradient conditions for deep proteome discovery in a complex protein matrix,” International Journal of Molecular Sciences, 2022, 23: 11714, doi.org/10.3390/ijms231911714.

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Mohamed Bayati, Hsin-Yeh Hsieh, Shu-Yu Hsu, Chenhui Li, Elizabeth Rogers, Anthony Belenchia, Jessica Klutts, Sally Zemmer, Melissa Reynolds, Elizabeth Semkiw, Hwei-Yiing Johnson, Trevor Foley, Chris G. Wieberg, Jeff Wenzel, Terri Lyddon, Mary LePique, Clayton Rushford, Braxton Salcedo, Kara Young, Madalyn Graham, Reinier Suarez, Anarose Ford, Zhentian Lei, Lloyd Sumner, Brian P. Mooney, Xing Wei, C. Michael Greenlief, Marc C. Johnson, and Chung-Ho Lin, “Identification and quantification of bioactive compounds suppressing SARS-CoV-2 signals in wastewater-based epidemiology surveillance,” Water Research2022, 221: 118824, doi.org/10.1016/j.watres.2022.118824.

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Shanyan Chen, Heather Siedhoff, Hua Zhang, Pei Liu, Ashley Balderrama, Runting Li, Catherine Johnson, C. Michael Greenlief, Bastijn Koopmans, Timothy Hoffman, Jiankun Cui, Ralph G. DePalma, De-Pei Li, Zezong Gu, “Low-intensity blast induces acute glutamatergic hyperexcitability in mouse hippocampus leading to long-term learning deficits and altered expression of proteins involved in synaptic plasticity and serine protease inhibitors,” Neurobiology of Disease, 2022, 165: 105634, doi.org/10.1016/j.nbd.2022.105634.

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Xing Wei, Renee D. JiJi, Anahita Zare, Bryan Lada, Xiyang Li and C. Michael Greenlief, “Deep-UV Resonance Raman Spectroscopy of Hydrated and Dehydrated Model α-Helical Transmembrane Peptides in Liposomes,” Journal of Raman Spectroscopy2022, 53: 58-68, doi.org/10.1002/jrs.6552.

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Colleen L. Ray, James A. Gawenis, and C. Michael Greenlief, “A new method for olive oil authenticity screening using multivariate analysis of proton NMR spectra,” Molecules, 2022, 27: 213, doi.org/10.3390/molecules27010213.

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Colleen L. Ray, James A. Gawenis, Michael Harmata, and C. Michael Greenlief, “NMR internal standard shifts due to cyclodextrin inclusion complexes,” Magnetic Resonance in Chemistry, 2022, 60: 80-85, doi.org/10.1002/mrc.5180.

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Grace Y. Sun, Xue Geng, Tao Teng, Bo Yang, Michael K. Appenteng, C. Michael Greenlief, and James C. Lee, “Dynamic role of phospholipases A2 in health and diseases in the central nervous system,” Cells, 2021, 10: 2963, doi.org/10.3390/cells10112963.

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Michael K. Appenteng, Ritter Krueger, Mitch C. Johnson, Harrison Ingold, Richard Bell, Andrew L. Thomas, and C. Michael Greenlief, “Cyanogenic glycoside analysis in American elderberry,” Molecules, 2021, 23: 1384, doi.org/10.3390/molecules26051384 (invited article).

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Grace Y. Sun, Michael K. Appenteng, Runting Li, Taeseon Woo, Bo Yang, Chao Qin, Meixia Pan, Magdalena Cieślik, Jiankun Cui, Kevin L. Fritsche, Zezong Gu, Matthew Will, David Beversdorf, Agata Adamczyk, Xianlin Han, and C. Michael Greenlief, “Docosahexaenoic acid (DHA) supplementation alters phospholipid species and lipid peroxidation products in adult mouse brain, heart, and plasma,” NeuroMolecular Medicine, 2021, 23: 118-129, doi.org/10.1007/s12017-020-08616-0.

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