Education: 

BS, Emporia State University, 1983
PhD, The University of Texas at Austin, 1987

Professional Experience: 

Director, MU NMR Facility, University of Missouri, 2007-present
Director, Charles W. Gehrke Proteomics Center, University of Missouri, 2008-present; Co-Director 2001-2008
Faculty member, Interdisciplinary Neuroscience Program, University of Missouri; 2018 – present
Office of Research Faculty Fellow, University of Missouri, 2004-2008
Director, Chemistry Mass Spectrometry Facility, 1997-present
Associate Professor, University of Missouri, 1994-present
Assistant Professor, University of Missouri, 1989-1994
Postdoctoral Research Associate, IBM T. J. Watson Research Center, 1987-1989

Professional Activities (partial listing of current activities)

American Chemical Society:

• Secretary/Treasurer of local section 1992-93
• Treasurer for the 1993 Midwest Regional Meeting
• Program Chair for the 2003 Midwest Regional Meeting
• General Chair for the 2014 Midwest Regional Meeting
• Member of Midwest Region Board of Directors 1997-present, Board Secretary 2006, 2011, 2016; Board Chair-Elect 2007, 2012, 2017; Board Chair 2008, 2013, 2018
• 2016 E. Ann Nalley Midwest Award for Volunteer Service
• Member, National Committee on Minority Affairs (2020-2024)
• Member, ACS Scholars Committee (2020-2022)

Member, American Association for the Advancement of Science, American Society for Mass Spectrometry, Society for the Advancement of Chicanos and Native Americans in Science, Society for Neuroscience

Editorial Board, PLoS One (2018-present)
Editorial Board, Molecules (2019-present)
Topics Board Editor, International Journal of Molecular Sciences (2021-present)
Editorial Board, International Journal of Molecular Sciences (2019-2021)

Research Emphasis: 

Analytical and Physical Chemistry, biological mass spectrometry, separation of complex mixtures, quantitative proteomics, directed metabolite analysis.

Research: 

In my laboratory, experimental work is being conducted in the area of mass spectrometry and NMR. We develop new methods to determine the bioactive components from dietary botanical supplements. We are also interested in biological systems at the protein level, as well as, quantitatively measuring targeted metabolites in a variety of matrices.

Colleen L. Ray, James A. Gawenis, Madison Bylo, Jonny Pescaglia, and C. Michael Greenlief, “Detection of Vegetable Oil Adulteration in Pre-Grated Bovine Hard Cheeses Via ¹H NMR Spectroscopy,” Molecules, 2023, 28: 920, doi.org/10.3390/molecules28030920.

Colleen L. Ray, Madison Bylo, Jonny Pescaglia , James A. Gawenis, and C. Michael Greenlief, “Delta-8 tetrahydrocannabinol product impurities,” Molecules, 2022,  27: 6924, doi.org/10.3390/molecules27206924.

Xing Wei, Pei N. Liu, Brian P. Mooney, Thao Thi Nguyen, and C. Michael Greenlief, “A comprehensive study of gradient conditions for deep proteome discovery in a complex protein matrix,” International Journal of Molecular Sciences, 2022, 23: 11714, doi.org/10.3390/ijms231911714.

Mohamed Bayati, Hsin-Yeh Hsieh, Shu-Yu Hsu, Chenhui Li, Elizabeth Rogers, Anthony Belenchia, Jessica Klutts, Sally Zemmer, Melissa Reynolds, Elizabeth Semkiw, Hwei-Yiing Johnson, Trevor Foley, Chris G. Wieberg, Jeff Wenzel, Terri Lyddon, Mary LePique, Clayton Rushford, Braxton Salcedo, Kara Young, Madalyn Graham, Reinier Suarez, Anarose Ford, Zhentian Lei, Lloyd Sumner, Brian P. Mooney, Xing Wei, C. Michael Greenlief, Marc C. Johnson, and Chung-Ho Lin, “Identification and quantification of bioactive compounds suppressing SARS-CoV-2 signals in wastewater-based epidemiology surveillance,” Water Research, 2022, 221: 118824, doi.org/10.1016/j.watres.2022.118824.

Shanyan Chen, Heather Siedhoff, Hua Zhang, Pei Liu, Ashley Balderrama, Runting Li, Catherine Johnson, C. Michael Greenlief, Bastijn Koopmans, Timothy Hoffman, Jiankun Cui, Ralph G. DePalma, De-Pei Li, Zezong Gu, “Low-intensity blast induces acute glutamatergic hyperexcitability in mouse hippocampus leading to long-term learning deficits and altered expression of proteins involved in synaptic plasticity and serine protease inhibitors,” Neurobiology of Disease, 2022, 165: 105634, doi.org/10.1016/j.nbd.2022.105634.

Xing Wei, Renee D. JiJi, Anahita Zare, Bryan Lada, Xiyang Li and C. Michael Greenlief, “Deep-UV Resonance Raman Spectroscopy of Hydrated and Dehydrated Model α-Helical Transmembrane Peptides in Liposomes,” Journal of Raman Spectroscopy, 2022, 53: 58-68, doi.org/10.1002/jrs.6552.

Colleen L. Ray, James A. Gawenis, and C. Michael Greenlief, “A new method for olive oil authenticity screening using multivariate analysis of proton NMR spectra,” Molecules, 2022, 27: 213, doi.org/10.3390/molecules27010213.

Colleen L. Ray, James A. Gawenis, Michael Harmata, and C. Michael Greenlief, “NMR internal standard shifts due to cyclodextrin inclusion complexes,” Magnetic Resonance in Chemistry, 2022, 60: 80-85, doi.org/10.1002/mrc.5180.

Grace Y. Sun, Xue Geng, Tao Teng, Bo Yang, Michael K. Appenteng, C. Michael Greenlief, and James C. Lee, “Dynamic role of phospholipases A2 in health and diseases in the central nervous system,” Cells, 2021, 10: 2963, doi.org/10.3390/cells10112963.

Michael K. Appenteng, Ritter Krueger, Mitch C. Johnson, Harrison Ingold, Richard Bell, Andrew L. Thomas, and C. Michael Greenlief, “Cyanogenic glycoside analysis in American elderberry,” Molecules, 2021, 23: 1384, doi.org/10.3390/molecules26051384 (invited article).

Grace Y. Sun, Michael K. Appenteng, Runting Li, Taeseon Woo, Bo Yang, Chao Qin, Meixia Pan, Magdalena Cieślik, Jiankun Cui, Kevin L. Fritsche, Zezong Gu, Matthew Will, David Beversdorf, Agata Adamczyk, Xianlin Han, and C. Michael Greenlief, “Docosahexaenoic acid (DHA) supplementation alters phospholipid species and lipid peroxidation products in adult mouse brain, heart, and plasma,” NeuroMolecular Medicine, 2021, 23: 118-129, doi.org/10.1007/s12017-020-08616-0.

Bo Yang, Runting Li, Pei N. Liu, Xue Geng, Brian P. Mooney, Chen Chen, Jianlin Cheng, Kevin L. Fritsche, David Q. Beversdorf, James C. Lee, Grace Y. Sun and C. Michael Greenlief, "Quantitative proteomics reveals docosahexaenoic acid-mediated neuroprotective effects in lipopolysaccharide-stimulated microglial cells," Journal of Proteome Research, 2020, 19: 2236-2246, doi.org/10.1021/acs.jproteome.9b00792.

Garrett Ungerer, Jiankun Cui, Tina Ndam, Mikeala Bekemeier, Hailong Song, Runting Li, Heather R. Siedhoff, Bo Yang, Michael K. Appenteng, C. Michael Greenlief, Dennis K. Miller, Grace Y. Sun, William R. Folk, and Zezong Gu, “Harpagophytum procumbens extract ameliorates allodynia and modulates oxidative and antioxidant stress pathways in a rat model of spinal cord injury,” NeuroMolecular Medicine, 2020, 22: 278-292, doi.org/10.1007/s12017-019-08585-z.

Hailong Song, Jiankun Cui, Valeri V. Mossine, C. Michael Greenlief, Kevin Fritsche, Grace Y. Sun, and Zezong Gu, “Bioactive components from garlic on brain resiliency against neuroinflammation and neurodegeneration (Review),” Experimental and Therapeutic Medicine, 2020, 19: 1554-1559, doi.org/10.3892/etm.2019.8389.

Xue Geng, Bo Yang, Runting Li, Tao Teng, Mary Jo Ladu, Grace Y. Sun, C. Michael Greenlief, and James C. Lee, “Effects of Docosahexaenoic Acid and its Peroxidation Product on Amyloid-β Peptide-Stimulated Microglia,” Molecular Neurobiology, 2020, 57: 1085-1098, doi.org/10.1007/s12035-019-01805-4.

Danh C. Vu, Jihyun Park, Van K. Ho, Lloyd W. Sumner, Zhentian Lei, C. Michael Greenlief, Brian Mooney, Mark V. Coggeshall, and Chung-Ho Lin, “Identifying Health-promoting Bioactive Phenolics in Black Walnut Using a Cloud-based Metabolomics Platform,” Journal of Food Measurement and Characterization, 2020, 14: 770-777, doi.org/10.1007/s11694-019-00325-y.

Bo Yang, Kevin L. Fritsche, David Q. Beversdorf, Zezong Gu, James C. Lee, William R. Folk, C. Michael Greenlief, and Grace Y. Sun, “Yin-Yang mechanisms regulating lipid peroxidation of docosahexaenoic acid and arachidonic acid in the central nervous system,” Frontiers in Neurology, 2019, 10: 642, doi.org/10.3389/fneur.2019.00642.

Bo Yang, Andrew L. Thomas, and C. Michael Greenlief, “Comparative proteomic analysis unveils critical pathways underlying the role of nitrogen fertilizer treatment in American elderberry,” Proteomes, 2019, 7: 10, doi:10.3390/proteomes7010010.

Bo Yang, Runting Li, Taeseon Woo, Jimmy D. Browning, Jr., Hailong Song, Zezong Gu, Jiankun Cui, James C. Lee, Kevin L. Fritsche, David Q. Beversdorf, Grace Y. Sun and C. Michael Greenlief, “Maternal dietary docosahexaenoic acid alters lipid peroxidation products and (n-3)/(n-6) fatty acid balance in offspring mice,” Metabolites, 2019, 9: 40, doi.org/10.3390/metabo9030040.

Grace Y. Sun, Runting Li, Bo Yang, Kevin L. Fritsche, David Q. Beversdorf, Dennis Lubahn, Xue Geng, James C. Lee, and C. Michael Greenlief, “Quercetin potentiates docosahexaenoic acid to suppress lipopolysaccharide-induced oxidative and inflammatory responses in BV-2 microglial cells,” International Journal of Molecular Sciences, 2019, 20: 932, doi.org/10.3390/ijms20040932.

Hailong Song, Hui Zhou, Zhe Qu, Jie Hou, Weilong Chen, Weiwu Cai, Qiong Cheng, Dennis Y Chuang, Shanyan Chen, Shuwei Li, Jilong Li, Jianlin Cheng, C. Michael Greenlief, Yuan Lu, Agnes Simonyi, Grace Y Sun, Chenghan Wu, Jiankun Cui, and Zezong Gu, “From Analysis of Ischemic Mouse Brain Proteome to Identification of Human Serum Clusterin as a Potential Biomarker for Severity of Acute Ischemic Stroke,” Translational Stroke Research, 2019, 10: 546-556, doi.org/10.1007/s12975-018-0675-2.